{"id":527,"date":"2022-03-14T10:12:33","date_gmt":"2022-03-14T02:12:33","guid":{"rendered":"http:\/\/www.hanzhong123.com\/?p=527"},"modified":"2022-03-14T10:13:30","modified_gmt":"2022-03-14T02:13:30","slug":"%e5%8b%bf%e5%82%ac%ef%bc%81%e4%b8%80%e4%bb%bd%e6%a0%b8%e9%85%b8%e6%a3%80%e6%b5%8b%e6%8a%a5%e5%91%8a%e7%9a%84%e8%af%9e%e7%94%9f%e4%b8%8d%e7%ae%80%e5%8d%95","status":"publish","type":"post","link":"http:\/\/www.hanzhong123.com\/527.html","title":{"rendered":"\u52ff\u50ac\uff01\u4e00\u4efd\u6838\u9178\u68c0\u6d4b\u62a5\u544a\u7684\u8bde\u751f\u4e0d\u7b80\u5355"},"content":{"rendered":"
\u201c\u5965\u5bc6\u514b\u620e\u201d\u6765\u88ad<\/p>\n
\u4e00\u4efd\u6838\u9178\u68c0\u6d4b\u9634\u6027\u62a5\u544a<\/p>\n
\u6210\u4e3a\u5fc5\u8981\u7684\u201c\u901a\u884c\u8bc1\u201d<\/p>\n
\u7ed3\u679c\u5c31\u51fa\u73b0\u4e86\u4ee5\u4e0b\u60c5\u666f<\/p>\n
<\/p>\n
<\/p>\n
<\/p>\n
<\/p>\n
\u5f88\u591a\u4eba\u4ee5\u4e3a<\/p>\n
\u6838\u9178\u68c0\u6d4b\u5c31\u50cf\u8840\u5e38\u89c4\u4e00\u6837<\/p>\n
\u53d6\u6807\u672c<\/p>\n
\u25bd<\/p>\n
\u653e\u4eea\u5668\u4e0a\u68c0\u6d4b<\/p>\n
\u25bd<\/p>\n
\u5f88\u5feb\u51fa\u7ed3\u679c<\/p>\n
\u7ed3\u679c\u5374\u53d1\u73b0\u2014\u2014<\/p>\n
<\/p>\n
\u5230\u5e95\u662f\u600e\u4e48\u56de\u4e8b\uff1f<\/p>\n
\u4e13\u4e1a\u4eba\u58eb\u7ec8\u4e8e\u505a\u51fa\u4e86\u89e3\u7b54\u2014\u2014<\/p>\n
\u771f\u6b63\u7684\u6838\u9178\u68c0\u6d4b\uff0c\u6ca1\u4f60\u60f3\u7684\u90a3\u4e48\u7b80\u5355\uff01<\/p>\n
<\/p>\n
Step 1 \u6807\u672c\u91c7\u96c6<\/p>\n
<\/p>\n
\u3000\u3000\u8fd9\u4e00\u6b65\u5927\u5bb6\u90fd\u61c2\uff0c\u201c\u6345\u55d3\u5b50\u773c\u513f\u201d\u201c\u6345\u9f3b\u5b50\u773c\u513f\u201d\u3002<\/p>\n
\u3000\u3000\u91c7\u96c6\u540e\u5c06\u62ed\u5b50\u5934\u6d78\u5165\u75c5\u6bd2\u4fdd\u5b58\u6db2\u4e2d\u4fdd\u5b58\uff0c\u5168\u57ce\u5927\u7b5b\u67e5\u4e2d\u901a\u5e38\u662f10\u4e2a\u62ed\u5b50\u4e00\u7ec4\u8fdb\u884c\u4fdd\u5b58\u3002<\/p>\n
Step 2 \u96c6\u5408\u8f6c\u8fd0<\/p>\n
<\/p>\n
\u3000\u3000\u5728\u5168\u57ce\u5927\u7b5b\u67e5\u4e2d\uff0c\u6838\u9178\u91c7\u6837\u5730\u70b9\u901a\u5e38\u662f\u793e\u533a\u3001\u5b66\u6821\u7b49\u4e34\u65f6\u91c7\u6837\u70b9\uff0c\u800c\u68c0\u6d4b\u70b9\u662f\u6709\u4e25\u683c\u7684\u751f\u7269\u5b89\u5168\u548c\u8d28\u91cf\u8981\u6c42\u7684\u5b9e\u9a8c\u5ba4\u3002\u56e0\u6b64\uff0c\u9f3b\u3001\u54bd\u62ed\u5b50\u5728\u91c7\u6837\u70b9\u96c6\u5408\u540e\uff0c\u88ab\u201c\u5168\u526f\u6b66\u88c5\u201d\u9001\u5f80\u5b9e\u9a8c\u5ba4\u3002<\/p>\n
Step 3 \u6838\u6536\u6d88\u6bd2<\/p>\n
<\/p>\n
\u3000\u3000\u5728\u4e25\u5bc6\u4fdd\u62a4\u4e0b\uff0c\u6807\u672c\u88ab\u201c\u62a4\u9001\u201d\u5230\u68c0\u6d4b\u5b9e\u9a8c\u5ba4\uff0c\u7531\u63a5\u6536\u4eba\u5458\u767b\u8bb0\u3001\u6838\u5bf9\u3002<\/p>\n
<\/p>\n
\u3000\u3000\u5168\u65b9\u4f4d\u6d88\u6bd2\uff0c\u51c6\u5907\u8fdb\u5165\u68c0\u6d4b\u73af\u8282\u3002<\/p>\n
<\/p>\n
Step 4 \u5168\u9762\u9632\u62a4<\/p>\n
<\/p>\n
\u3000\u3000\u8fdb\u5165\u6837\u672c\u5904\u7406\u533a\u57df\u524d\uff0c\u68c0\u6d4b\u4eba\u5458\u9700\u505a\u5230100%\u7684\u5b89\u5168\u9632\u62a4\u3002<\/p>\n
Step 5 \u4fe1\u606f\u5f55\u5165<\/p>\n
<\/p>\n
\u3000\u3000\u5b8c\u6210\u5168\u9762\u9632\u62a4\u7684\u5de5\u4f5c\u4eba\u5458\u8fdb\u5165\u6837\u672c\u5904\u7406\u533a\uff0c\u5c06\u6837\u672c\u5728\u751f\u7269\u5b89\u5168\u67dc\u5185\u6253\u5f00\uff0c\u53d6\u51fa\u6837\u672c\u6d88\u6bd2\u5e76\u5f55\u5165\u4fe1\u606f\u7cfb\u7edf\u3002\u76ee\u524d\u4fe1\u606f\u5f55\u5165\u6709\u4e24\u79cd\uff0c\u4e00\u79cd\u662f\u6761\u7801\u5f55\u5165\uff0c\u9700\u8981\u201c\u6ef4\u201d\u4e00\u58f0\u89e3\u51b3\uff1b\u53e6\u4e00\u79cd\u662f\u624b\u5de5\u586b\u5199\u7684\u5355\u5b50\uff0c\u9700\u8981\u6328\u4e2a\u6458\u5f55\u5230\u7cfb\u7edf\u4e2d\u3002<\/p>\n
Step 6 \u8bd5\u5242\u914d\u5236<\/p>\n
<\/p>\n
\u3000\u3000\u6839\u636e\u5f85\u68c0\u6837\u672c\u6570\u91cf\u914d\u7f6e\u597d\u6838\u9178\u68c0\u6d4b\u5fc5\u5907\u7684\u8bd5\u5242\u548c\u8f85\u52a9\u6750\u6599\uff0c\u7cbe\u51c6\u914d\u5236\u548c\u5206\u88c5\u53cd\u5e94\u4f53\u7cfb\u3002\u4e3a\u4e86\u63d0\u9ad8\u6548\u7387\uff0c\u8fd9\u4e00\u6b65\u548c\u63a5\u4e0b\u6765\u7684\u4e24\u6b65\u901a\u5e38\u7531\u4e0d\u540c\u7684\u68c0\u6d4b\u4eba\u5458\u540c\u65f6\u8fdb\u884c\u3002<\/p>\n
Step 7 \u6838\u9178\u63d0\u53d6<\/p>\n
\u2460 \u88c2\u89e3<\/p>\n
\u3000\u3000\u5728\u8fdb\u884c\u6838\u9178\u68c0\u6d4b\u524d\uff0c\u5fc5\u987b\u5148\u7528\u80cd\u76d0\u628a\u82b1\u82b1\u7eff\u7eff\u7684\u86cb\u767d\u8d28\u201c\u9a6c\u7532\u201d\u7ed9\u8131\u4e86\uff0c\u624d\u80fd\u5c06\u75c5\u6bd2\u7684\u6838\u9178\u91ca\u653e\u51fa\u6765\uff0c\u9732\u51fa\u75c5\u6bd2\u7684\u201c\u771f\u9762\u76ee\u201d\u3002<\/p>\n
<\/p>\n
\u2461 \u7ed3\u5408<\/p>\n
\u3000\u3000\u52a0\u5165\u78c1\u73e0\u4e0e\u6838\u9178\u7ed3\u5408\u3002\u4e0e\u5404\u79cd\u7ec6\u80de\u788e\u7247\u3001\u86cb\u767d\u8d28\u8bf4\u201c\u62dc\u62dc\u201d\u5566\uff01<\/p>\n
\u3000\u3000\u201c\u6211\u4eec\u7684\u76ee\u6807\u662f\uff1f\u201d\u201c\u6293\u4f4f\u6838\u9178\uff01\u201d<\/p>\n
\u2462 \u6d17\u6da4<\/p>\n
\u3000\u3000\u7ed3\u5408\u4e00\u6b21\u5c31\u80fd\u6293\u5230\u6838\u9178\u4e86\uff1f\u54ea\u6709\u90a3\u4e48\u8f7b\u677e\u54e6\uff01\u6293\u662f\u6293\u5230\u4e86\uff0c\u4e0d\u8fc7\u8fd8\u662f\u6709\u5c11\u91cf\u7684\u788e\u7247\u3001\u86cb\u767d\u8ddf\u7740\u6838\u9178\u4e00\u8d77\u88ab\u9ecf\u4e0a\u4e86\u3002\u5982\u4f55\u63d0\u9ad8\u6838\u9178\u7684\u7eaf\u5ea6\uff1f\u6211\u4eec\u6709\u4e24\u5927\u6cd5\u5b9d\uff1a\u52a0\u76d0\uff0c\u52a0\u9178\u3002<\/p>\n
\u3000\u3000 \u201c\u5438\u9644\u6838\u9178-\u9ad8\u76d0\u4f4epH\u201d\u3001\u201c\u6d17\u8131\u6838\u9178-\u4f4e\u76d0\u9ad8pH\u201d\uff0c\u53cd\u590d\u4e24\u4e09\u6b21\u7684\u5438\u9644\u3001\u6d17\u8131\uff0c\u5b9e\u73b0\u6838\u9178\u7684\u63d0\u53d6\u7eaf\u5316\u3002\u5269\u4e0b\u7684\u5c31\u662f\u201c\u8d64\u88f8\u88f8\u201d\u7684\u6838\u9178\u5566\u3002<\/p>\n
<\/p>\n
Step 8 \u6838\u9178\u6269\u589e<\/p>\n
\u3000\u3000\u65b0\u51a0\u75c5\u6bd2\u6838\u9178\u68c0\u6d4b\u5e38\u89c4\u7b5b\u67e5\u91c7\u7528\u7684\u662f\u5b9e\u65f6\u8367\u5149PCR\u65b9\u6cd5\u3002PCR\u6280\u672f\u4e8e1983\u5e74\u7531\u7f8e\u56fd\u8457\u540d\u5316\u5b66\u5bb6\u51ef\u5229\u00b7\u7a46\u5229\u65af\uff08Kary Banks Mullis\uff09\u53d1\u660e\uff0c\u5e76\u83b7\u5f971993\u5e74\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u3002PCR\u6280\u672f\u662f\u901a\u8fc7\u6a21\u62df\u751f\u7269\u4f53\u4f53\u5185DNA\u590d\u5236\u7684\u65b9\u5f0f\uff0c\u5728\u4f53\u5916\u9009\u62e9\u6027\u5730\u5c06DNA\u67d0\u4e2a\u7279\u6b8a\u533a\u57df\u6269\u589e\u51fa\u6765\u7684\u6280\u672f\u3002<\/p>\n
\u3000\u3000PCR\u53cd\u5e94\u5c31\u50cf\u4e00\u4e2a\u5927\u578b\u88c5\u914d\u8f66\u95f4\u3002\u60f3\u8981\u6269\u589e\u67d0\u4e2a\u75c5\u6bd2\u7247\u6bb5\uff0c\u9996\u5148\u6839\u636e\u5b83\u7684\u7279\u70b9\u8bbe\u8ba1\u4e00\u5bf9\u5f15\u7269\u3002\u5f15\u7269\u5c31\u662f\u6700\u6709\u529b\u7684\u8bc6\u522b\u5e76\u5b9a\u4f4d\u7684\u201c\u6293\u624b\u201d\uff0c\u5177\u6709\u201c\u6307\u54ea\u6253\u54ea\u201d\u7684\u7cbe\u786e\u6027\u3002\u5728\u4e00\u7fa4\u75c5\u6bd2\u6838\u9178\u4e2d\uff0c\u60f3\u6293\u65b0\u51a0\u8fd8\u662f\u6d41\u611f\uff0c\u6291\u6216\u662f\u9f3b\u75c5\u6bd2\u3001\u547c\u5438\u9053\u5408\u80de\u75c5\u6bd2\u7b49\uff0c\u53ea\u6709\u4f60\u60f3\u4e0d\u5230\uff0c\u6ca1\u6709\u4ed6\u6293\u4e0d\u5230\u3002<\/p>\n
<\/p>\n
\u3000\u3000\u5f53\u4ed6\u8bc6\u522b\u5230\u65b0\u51a0\u75c5\u6bd2\u7684\u201c\u7279\u70b9\u201d\u2014\u2014\u7279\u5f02\u6027\u7684\u7247\u6bb5\uff0c\u9a6c\u4e0a\u4e00\u524d\u4e00\u540e\u201c\u6293\u6355\u201d\uff0c\u518d\u6dfb\u52a0dNTP\u3001Mg2+\u3001Taq\u9176\u7b49\u539f\u6750\u6599\uff0c\u8c03\u8282\u4e0d\u540c\u7684\u6e29\u5ea6\uff0c\u5386\u7ecf\u201c\u53d8\u6027-\u9000\u706b-\u5ef6\u4f38\u201d\u4e09\u5927\u6b65\uff0c\u5b8c\u6210\u75c5\u6bd2\u6838\u9178\u7ec4\u88c5\u3002\u6bcf\u6269\u589e\u4e00\u6b21\uff0cDNA\u4ea7\u91cf\u5448\u6307\u6570\u589e\u957f\u3002\u65b0\u51a0\u75c5\u6bd2\u6269\u589e\u4e00\u822c\u9700\u898140\u4e2a\u5faa\u73af\uff0c\u7406\u8bba\u4e0a1\u4e2a\u62f7\u8d1d\u53ef\u4ee5\u5f97\u5230240=1,099,511,627,776\u4e2a\u62f7\u8d1d\u3002\u5373\u4f7f\u6837\u672c\u4e2d\u75c5\u6bd2\u542b\u91cf\u5f88\u4f4e\uff0c\u90fd\u53ef\u4ee5\u901a\u8fc7PCR\u6269\u589e\u68c0\u6d4b\u51fa\u6765\u3002\u56e0\u6b64PCR\u53cd\u5e94\u6709\u9ad8\u7279\u5f02\u6027\u548c\u9ad8\u7075\u654f\u5ea6\u7684\u7279\u70b9\u3002<\/p>\n
<\/p>\n
\u3000\u3000240\u4e2a\u6838\u9178\u7247\u6bb5\u62f7\u8d1d\uff0c\u6570\u91cf\u662f\u5f88\u591a\uff0c\u4f46\u65e0\u8272\u65e0\u5473\u53c8\u770b\u4e0d\u89c1\uff0c\u201c\u654c\u5728\u6697\u5904\u201d\u600e\u4e48\u529e\uff1f\u90a3\u6211\u4eec\u5c31\u8ba9\u4ed6\u5728\u201c\u660e\u5904\u201d\uff01\u5b9e\u65f6\u8367\u5149PCR\u53cd\u5e94\u5c31\u662f\u5728PCR\u53cd\u5e94\u57fa\u7840\u4e0a\u52a0\u5165\u63a2\u9488\uff0c\u6bcf\u6269\u589e\u51fa\u76ee\u6807\u7247\u6bb5\u5373\u4f1a\u53d1\u51fa\u8367\u5149\uff0c\u88ab\u8367\u5149\u63a2\u6d4b\u5668\u6355\u83b7\u3002<\/p>\n
<\/p>\n
\u3000\u3000\u968f\u7740\u6269\u589e\u6b21\u6570\u7684\u589e\u52a0\uff0c\u8367\u5149\u91cf\u7d2f\u79ef\uff0c\u5c31\u662f\u6211\u4eec\u770b\u5230\u7684\u66f2\u7ebf\u56fe\u4e86\uff0c\u6211\u4eec\u5c31\u662f\u901a\u8fc7\u5b83\u6765\u5224\u65ad\u6709\u6ca1\u6709\u65b0\u51a0\u75c5\u6bd2\u7684\u3002<\/p>\n
<\/p>\n
\u3000\u3000\u201c\u54a6\uff0c\u533b\u751f\uff0c\u4e3a\u5565\u9633\u6027\u66f2\u7ebf\u56fe\u548c\u9634\u6027\u66f2\u7ebf\u56fe\u91cc\u9762\u90fd\u6709\u4e2a\u9633\u6027\u7684\u66f2\u7ebf\u5583\uff1f\u662f\u4e0d\u662f\u9634\u6027\u88ab\u6c61\u67d3\u4e86\u54e6\uff1f\u201d<\/p>\n
\u3000\u3000\u201c\u83ab\u614c\uff0c\u90a3\u4e2a\u662f\u4eba\u7c7b\u7ba1\u5bb6\u57fa\u56e0\uff0c\u5c31\u662f\u4eba\u7c7b\u6240\u6709\u7ec6\u80de\u90fd\u7a33\u5b9a\u8868\u8fbe\u7684\u57fa\u56e0\u3002\u201d<\/p>\n
\u3000\u3000\u201c\u6211\u662f\u67e5\u65b0\u51a0\u75c5\u6bd2\uff0c\u8fd9\u4e2a\u4ec0\u4e48\u201c\u7ba1\u5bb6\u201d\u662f\u6765\u5e72\u5565\u5462\uff1f\u201d<\/p>\n
\u3000\u3000\u201c\u8fd9\u4e2a\u662f\u7528\u6765\u76d1\u6d4b\u91c7\u6837\u3001\u8fd0\u8f93\u4ee5\u53ca\u68c0\u6d4b\u8fc7\u7a0b\u7684\uff01\u201d<\/p>\n
\u3000\u3000\u201c\u662f\u4e0d\u662f\u7ba1\u5bb6\u57fa\u56e0\u9633\u6027\u5c31\u4ee3\u8868\u91c7\u6837\u90e8\u4f4d\u6ca1\u95ee\u9898\u5462\uff1f\u201d<\/p>\n
\u3000\u3000\u201c\u8fd9\u53ef\u4e0d\u4e00\u5b9a\uff0c\u7ba1\u5bb6\u57fa\u56e0\u561b\uff0c\u53ea\u80fd\u4ee3\u8868\u91c7\u5230\u4eba\u4f53\u7ec4\u7ec7\u4e86\uff0c\u81f3\u4e8e\u662f\u5426\u91c7\u5230\u75c5\u6bd2\u6f5c\u4f0f\u90e8\u4f4d\uff0c\u8fd9\u53ef\u4e0d\u4e00\u5b9a\u3002\u201d<\/p>\n
<\/p>\n
\u3000\u3000\u68c0\u6d4b\u8fc7\u7a0b\u4e2d\u4e0d\u80fd\u505c\u4e0b\u6765\u6dfb\u52a0\u65b0\u6807\u672c\uff0c\u5fc5\u987b\u7b49\u5f85\u8fd9\u4e00\u6279\u6269\u589e\u5b8c\u6210\uff0c\u624d\u80fd\u8fdb\u884c\u4e0b\u4e00\u6279\u7684\u6269\u589e\uff0c\u8fd9\u4e5f\u662f\u6807\u672c\u4e0d\u80fd\u968f\u5230\u968f\u68c0\u7684\u539f\u56e0\u4e4b\u4e00\u3002\u4e00\u4e2a\u5927\u578b\u7684\u5b9e\u9a8c\u5ba4\u5185\uff0c\u6838\u9178\u6269\u589e\u8bbe\u5907\u591a\u8fbe\u4e0a\u767e\u53f0\uff0c\u540c\u65f6\u4e0d\u95f4\u65ad\u5de5\u4f5c\u7684\u68c0\u6d4b\u4eba\u5458\u6709\u6570\u767e\u4eba\u3002<\/p>\n
Step 9 \u68c0\u540e\u5904\u7406<\/p>\n
<\/p>\n
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Step 10 \u7ed3\u679c\u51fa\u7089<\/p>\n
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\u6765\u6e90\uff1a\u6c49\u53f0\u75be\u63a7<\/p>\n","protected":false},"excerpt":{"rendered":"
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